LncRNA SNHG1 and RNA binding protein hnRNPL form a complex and coregulate CDH1 to boost the growth and metastasis of prostate cancer

Abstract The interaction between LncRNA and RNA-binding protein (RBPs) plays an essential role in the regulation over malignant progression of tumors. Previous studies on mechanism SNHG1 , emerging lncRNA, have primarily focused competing endogenous RNA (ceRNA) mechanism. Nevertheless, underlying RBPs tumors remains to be explored, especially prostate cancer (PCa). expression profiles PCa were determined through analysis TCGA data tissue microarray at level. Gain- loss-of-function experiments performed investigate biological initiation progression. RNA-seq, immunoblotting, pull-down immunoprecipitation analyses utilized clarify potential pathways with which might involved. Finally, rescue carried out further confirm this We found that was dominantly expressed nuclei cells significantly upregulated patients. higher level dramatically correlated tumor metastasis patient survival. Functionally, overexpression induced epithelial–mesenchymal transition (EMT), accompanied by down-regulation epithelial marker, E-cadherin up-regulation mesenchymal vimentin . Increased proliferation migration, as well accelerated xenograft growth, observed -overexpressing cells, while opposite effects achieved -silenced cells. Mechanistically, competitively interacted hnRNPL impair translation thus activating effect EMT pathway, eventually promoting PCa. Our findings demonstrate is a positive regulator activation - -CDH1 axis. may serve novel therapeutic target for